| Solution Information | help | |
| Enzyme: | RAC-alpha serine/threonine-protein kinase | |
| inhibitor: | BDBM108444 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | 7.5 | |
| Temp. Prep.: | 80 | |
| Comments: | Thermal Shift Assay | |
| pH Buffer 1: | Tris | |
| Concentration: | 50 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 2: | BSA | |
| Concentration: | 0.02 mg/mL | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 3: | MgCl2 | |
| Concentration: | 10 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 4: | EGTA | |
| Concentration: | 1 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 5: | glycerol | |
| Concentration: | 10% | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 6: | Na3VO4 | |
| Concentration: | 0.2 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 7: | β-glycerophosphate | |
| Concentration: | 0.1 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 8: | NaF | |
| Concentration: | 0.2 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 9: | Tris-HCl | |
| Concentration: | 25 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 10: | NaCl | |
| Concentration: | 100 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 11: | DTT | |
| Concentration: | 5 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
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